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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Thromb Haemost. 2018 Apr 1;16(5):919–932. doi: 10.1111/jth.13988

Figure 5. Decreased nidogen and increased fibrin content in Plg-RKT−/− lactating mammary glands.

Figure 5

Thoracic mammary glands were harvested from Plg-RKT+/+ and Plg-RKT−/− female mice two days postpartum. Glands were lysed and electrophoresed on 4–12% gradient gels under reducing conditions and western blotted with anti-entactin/nidogen and anti-βactin (A) or electrophoresed under non-reducing conditions and western blotted with anti-fibrin(ogen) and anti-βactin. (C). Gels were scanned and quantified densitometrically using LI-COR software (B (***=P=0.0007, n=2–3) D (*=P=0.027, n=3). Total fibrin(ogen) content of glands was determined by ELISA (E) (**P=0.0016, n=8).