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. Author manuscript; available in PMC: 2019 May 1.
Published in final edited form as: J Thromb Haemost. 2018 Apr 1;16(5):919–932. doi: 10.1111/jth.13988

Figure 7. Plg-RKT deletion decreases epithelial cell proliferation and increases epithelial cell apoptosis.

Figure 7

Mammary glands were harvested two days postpartum from Plg-RKT+/+ and Plg-RKT−/− female mice. Frozen sections of abdominal mammary glands were immunostained with antibodies against Ser28-phosphorylated histone H3 (Pho-HIS-A568) and Dapi (shown in white for greater clarity). Boxes defined by dashed lines are the areas enlarged in right panels. Confocal images were captured using a Zeiss 710 Laser Scanning Confocal Microscope running the Zen 2016 software. 8 bit optical image section slices (sampled at 0.3μm intervals) were collected as 1024×1024 images, converted to maximum intensity projections for 2D analysis then imported into: Image Pro Premier, Image J and LSM Examiner software for further processing and quantitative analysis. (A). Thoracic glands were lysed and electrophoresed on 4–12% gradient gels under reducing conditions and western blotted with anti-Mcl-1 and anti-βactin (B) or anti-active (cleaved) caspase 3 and anti-βactin (C).