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Calibration assays for human IgE antibodies spiked in calf serum: A) background-subtracted SPRi responses for IgEs captured on MB-Ab2 and injected into anti-IgE immobilized SPRi spots. Calibration plots of human IgE antibodies measured on SPRi spots coated with B) anti-IgE, C) Ara h2–3, D) Ara h2–5, E) AFG, and F) BXG. Error bars represent standard deviations for n=4. μRIU: micro-refractive index unit.
