Figure 2.

SSCs and EL4 cells shape and cytotoxicity analyses.

Notes: (A) A colony of SSCs (bar = 100 μm). (B) EL4 cell line (bar = 100 μm). (C) Cytotoxicity study of 0.5, 1, and 2 mg/mL of CDDP/PLGA and blank NPs (0.5, 1, and 2 mg/mL) and 10 μg/mL of free cisplatin after 48-hour incubation with EL4 cells and SSCs as determined by the MTT assay (a): there is a significant difference between the experimental groups compared with the control and blank NPs group (p ≤ 0.05), and (b): there is a significant difference between the groups in relation to each other that received different doses of cisplatin-loaded PLGA NPs (p ≤ 0.05). (D) Comparison of 2 mg/mL concentration of CDDP/PLGA NP and free cisplatin on the cell lines. As shown by the calibration curve for the release of the drug, the results obtained are confirmed by the results of the evaluation of cell survival with MTT.

Abbreviations: SSCs, spermatogonial stem cells; CDDP, cis-diaminedichloroplatinum; PLGA, poly(lactic-co-glycolic acid); NPs, nanoparticles.