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. 2018 May 18;13:2943–2954. doi: 10.2147/IJN.S155052

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© 2018 Shabani et al. This work is published and licensed by Dove Medical Press Limited

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In vivo tumor histological examination and evaluation of decontamination of SSCs from EL4 cells after treatment with CDDP/PLGA NPs.

Notes: (A) Histological analysis of busulfan-treated control mice. (B) Normal seminiferous tubules are seen in the group that received EL4 cells and SSCs after treatment. (C) Histological section of tumor formed from EL4 cells alone stained with H&E (bar = 100 μm). Cell labeling of SSCs and transplantation. (D) A cluster of SSCs labeled with DiI, (E) cells labeled with DAPI, and (F) merged. Bar = 100 μm. (G–I) Colonization and proliferation of labeled injected cells (bar = 50 μm).

Abbreviations: SSCs, spermatogonial stem cell; CDDP, cis-diaminedichloroplatinum; PLGA, poly(lactic-co-glycolic acid); NPs, nanoparticles.