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. 2018 May 18;11:2945–2954. doi: 10.2147/OTT.S165436

Table 1.

Primers used in quantitative polymerase chain reaction (qPCR) experiments

mRNA Sequence
ABCB1 (ATP-binding cassette subfamily B member 1)
 Forward primer 5′-TCTGGACAAGCACTGAAAGATAAG-3′
 Reverse primer 5′-ACCTGCAAACTCTGAGCATACATA-3′
ABCC1 (ATP-binding cassette subfamily C member 1)
 Forward primer 5′-CCGTGTACTCCAACGCTGACAT-3′
 Reverse primer 5′-ATGCTGTGCGTGACCAAGATCC-3′
ABCC2 (ATP-binding cassette subfamily C member 2)
 Forward primer 5′-GCCAACTTGTGGCTGTGATAGG-3′
 Reverse primer 5′-ATCCAGGACTGCTGTGGGACAT-3′
ABCC3 (ATP-binding cassette subfamily C member 3)
 Forward primer 5′-GAGGAGAAAGCAGCCATTGGCA-3′
 Reverse primer 5′-TCCAATGGCAGCCGCACTTTGA-3′
ABCG2 (ATP-binding cassette subfamily G member 2)
 Forward primer 5′-GTTCTCAGCAGCTCTTCGGCTT-3′
 Reverse primer 5′-TCCTCCAGACACACCACGGATA-3′
ADRA2A (adrenoceptor α2A)
 Forward primer 5′-CCAGGGCACTCAGAAACC-3′
 Reverse primer 5′-AAACGACGACATAGAGGGACT-3′
ALDH1A1 (aldehyde dehydrogenase 1 family)
 Forward primer 5′-ACAGGGTCTACTGAGGTTG-3′
 Reverse primer 5′-CTCCACGAAGATGCGAGA-3′
Apo E (apolipoprotein E)
 Forward primer 5′-GAATTCGCCCCGCCTGGTACAC-3′
 Reverse primer 5′-TAAGCTTGGCACGGCTGTCCAAGGA-3′
COMT (catechol-O-methyltransferase)
 Forward primer 5′-TCCTTCTCAACTGCCATTCC-3′
 Reverse primer 5′-CCTTAGCGTTAGCGTCCGTC-3′
CYP1A2 (cytochrome P450 1A2)
 Forward primer 5′-CATAGCCTCAGACCCCACAT-3′
 Reverse primer 5′-ACATTAGCCACCGATTCCAC-3′
CYP2B6 (cytochrome P450 2B6)
 Forward primer 5′-TCTGGATGCCAATGGGGCACTGAA-3′
 Reverse primer 5′-GGGCGATGCCTTCACCAAGACAAATT-3′
CYP2C11 (cytochrome P450 2C11)
 Forward primer 5′-GAGGACCATTGAGGACCGTA-3′
 Reverse primer 5′-GAGCACAGCCCAGGATAAAG-3′
CYP2C19 (cytochrome P450 2C19)
 Forward primer 5′-AACTACTTCATGCCTTTCTC-3′
 Reverse primer 5′-GGTCCTTTGGGTCAATCAG-3′
CYP2C22 (cytochrome P450 2C22)
 Forward primer 5′-ATGGGGATGGGAAAGAGAAC-3′
 Reverse primer 5′-TGCTGGAAAATGACACTGGA-3′
CYP2C9 (cytochrome P450 2C9)
 Forward primer 5′-CTTGACACCACTCCAGTTGTC-3′
 Reverse primer 5′-AGATGGATAATGCCCCAGAG-3′
CYP2D6 (cytochrome P450 2D6)
 Forward primer 5′-CTTCACCTCCCTGCTGCAG-3′
 Reverse primer 5′-TCACCAGGAAAGCAAAGACA-3′
CYP3A1 (cytochrome P450 3A1)
 Forward primer 5′-AAATGCCTCTGTTTGCCATC-3′
 Reverse primer 5′-CTTTCCCCATAATCCCCACT-3′
CYP3A4 (cytochrome P450 3A4)
 Forward primer 5′-CCCCTGAAATTAAGCTTAGGAG-3′
 Reverse primer 5′-TAATTTGAGGTCTCTGGTGTTCTCA-3′
CYP3A5 (cytochrome P450 3A5)
 Forward primer 5′-CATGACTTAGTAGACAGATGAC-3′
 Reverse primer 5′-GGTCCAAACAGGGAAGAAATA-3′
CYP4A11 (cytochrome P450 4A11)
 Forward primer 5′-CTTGGTCATGACTCCTAAACAGAGT-3′
 Reverse primer 5′-AGCCTGTTTACCAATTCTCTTTCAC-3′
CYP4B1 (cytochrome P450 4B1)
 Forward primer 5′-GCCTCCTATCCCTGGACTCC-3′
 Reverse primer 5′-AGCCCCGAGGTGGCTCCTG-3′
DPYD (dihydropyrimidine dehydrogenase)
 Forward primer 5′-TGTTCGGACAGAGCAAGATG-3′
 Reverse primer 5′-CTTCAATCCGGCCATTTCTA-3′
HTR2A (5-hydroxytryptamine receptor 2A)
 Forward primer 5′-ATGTCCTCGGAGTGCTGTG-3′
 Reverse primer 5′-TAGCCACCCTGAGCCTATGT-3′
MTHFR (methylenetetrahydrofolate reductase)
 Forward primer 5′-GTGTCTGCGGGAGCCGATTTCAT-3′
 Reverse primer 5′-GGCGCTTCAGCACCTCTGTGG-3′
TPMT (thiopurine S-methyltransferase)
 Forward primer 5′-AGGCAGCTAGGGAAAAAGAAAGCTG-3′
 Reverse primer 5′-CAAGCCTTATAGCCTTACACCCAGG-3′
UGT1A1 (UDP glucuronosyltransferase family 1 member A1)
 Forward primer 5′-GGCCCATCATGCCCAATAT-3′
 Reverse primer 5′-TTCAAATCCTGGGATAGTGGATT-3′
UGT2B7 (UDP glucuronosyltransferase family 2 member B7)
 Forward primer 5′-CAAAGGAHCTAAACACCTTCGG-3′
 Reverse primer 5′-CCGTAGTGTTTTCTTCATTGCC-3′