Figure 3.

Neutrophils Produce IL-1β in a Microbe-Size-Dependent Manner

(A and B) WT mice treated with isotype control IgG or anti-Ly6G antibody prior to intratracheal infection with 10⁵ yeast-locked (YL) or WT C. albicans. Please also see Figure S4A.

(A) C. albicans burden in the lung 48 hr after infection. Mean ± SD of three animals per group. Statistics by two-way ANOVA followed by Sidak’s multiple comparison post-test.

(B) IL-1β mRNA in the lung normalized to HPRT1 expression and IL-1β protein expression in bronchoalveolar lavage 24 hr after infection. Each point represents one animal. Statistics by two-way ANOVA followed by Sidak’s multiple comparison post-test.

(C) Fraction and total number of lung neutrophils positive for intracellular IL-1β staining measured by FACS. Neutrophils were gated as live CD45⁺, CD11b⁺, Ly6G⁺ cells. Each point represents one animal. Statistics by one-way ANOVA followed by Tukey’s multiple comparison post-test. Please also see Figure S4C.

(D) IL-1β protein and mRNA in neutrophils isolated by negative selection from lungs of WT mice infected with 10⁵C. albicans YL or WT. Each point represents one animal. Statistics by one-way ANOVA followed by Tukey’s multiple comparison post-test.

^∗∗∗∗p < 0.0001, ^∗∗∗p < 0.001, ^∗∗p < 0.01, ^∗p < 0.05.