Figure 6. Serial histological changes of the injured nerves with HE staining (1st row), LFB staining (2nd row) and Masson staining (3rd row). Proliferation of inflammatory cells, oedema and swelling occurred at 3 days post-injury. The myelinated nerve fibres began to break down at 3 days post-injury and thinly myelinated fibres gradually proliferated since 6 weeks post-injury. The thickening and fibrosis of epineurium and perineurium started at 4 weeks post-injury and progressed during the observation period. HE, haematoxylin–eosin; LFB, Luxol Fast Blue.