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. Author manuscript; available in PMC: 2018 Jun 13.
Published in final edited form as: Nature. 2017 Dec 13;552(7685):404–409. doi: 10.1038/nature25144

Figure 1. Dynamic changes in DNA methylation during effector and memory CD8 T cell differentiation.

Figure 1

A) Analysis of on-off-on gene expression from published naïve, and LCMV-specific effector, and memory CD8 T cell microarray data sets6. B) Histogram analysis of CD62L protein expression on naïve, effector and memory P14 CD8 T cells following acute LCMV infection. C) Experimental setup and bisulfite sequencing DNA methylation analysis of the CD62L promoter from P14 CD8 T cell purified (>95% purity) at the indicated stages of differentiation. Each horizontal line represents an individual sequenced clone. Filled circles = methylated cytosine. Open circles = non-methylated cytosine. Representative post-sort purity and DNA methylation analysis of the CD62L proximal promoter CpG sites among D) terminal effector (TE) and memory precursor (MP) P14 CD8 T cells FACS purified 8 days after acute LCMV infection, or E) effector-memory (Tem) and central memory (Tcm) P14 CD8 T cells FACS purified 37 days after acute LCMV infection.