Skip to main content
. Author manuscript; available in PMC: 2018 May 23.
Published in final edited form as: Cancer Biol Ther. 2010 Jun 11;9(12):1065–1078. doi: 10.4161/cbt.9.12.11995

Figure 2.

Figure 2

Nm23-H1 reduces the transcriptional activity of the Cyclin D1 promoter in B cells and binding to the specific site in the promoter. (A) pGL2-Cyclin D1 promoter was co-transfected with increasing amounts of pA3M-Nm23-H1, the pCMV-Rlu promoter and 24 h post-transfection BJAB or DG75 or 293 cells were harvested for dual luciferase assay. Increasing amounts of Nm23-H1 showed a dose-dependent decrease of promoter activity. (B) Different truncation along with the full length Cyclin D1 was shown in the left top of the figure. Either the full length or the truncated one pGL2-Cyclin D1 promoter was co-transfected with pA3M-Nm23-H1 and the CMV-Rlu promoter and 24 h post-transfection BJAB or DG 75 or 293 cells were harvested for dual luciferase assay. The promoters lacking AP1 sites (mutant number 4, 5 and 6) showed a lack of downregulation activity in presence of Nm23-H1.