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. Author manuscript; available in PMC: 2018 May 23.
Published in final edited form as: Cancer Biol Ther. 2010 Jun 11;9(12):1065–1078. doi: 10.4161/cbt.9.12.11995

Figure 5.

Figure 5

Nm23-H1 specifically interacts with the DNA-binding domain of p53. (A) A schematic representation of Full length domains along with the different truncations. (B) In vitro-translated Nm23-H1 protein was 35S labelled and tested for binding with a number of truncated mutants of GST-p53 constructs mapping the binding region (Full length, 1–80, 100–300 and 300–393). Relative binding levels are shown in the bar diagrams. (C) Lysates from Nm23-H1 expressing BJAB and 293 cells were incubated with the GST-p53 FL and the results of the binding are shown.