Fig 1. Cell elongation and dynamics of FtsZ during the vegetative cell cycle of L. lactis.

(A) Cell length (in μm) was measured for 11 individual cells analyzed by time-lapse microscopy (representative example shown on the top) and the resulting length curves were aligned on the start of cell constriction (T₀ = 0 min). The cell cycle is separated in two phases without obvious transition between: i. cell elongation only and ii. combined elongation and division during and after constriction. (B) Time-lapse imaging of FtsZ-Ve during cell elongation and division. L. lactis cells expressing the FtsZ-Ve fluorescent protein (NZ3900 [pGIBLD031]) were grown on agar pads and visualized by phase contrast (PC, top row) and epifluorescence (FtsZ-Ve, lower row) microscopy (see also S3 Fig [Cell #1] and S1 and S2 Movies). Pictures were taken every 10 min. Observed structural changes of the FtsZ ring (green line) are schematically represented for different steps of the cell cycle. The shaded green band depicts the fuzzy aspect of FtsZ structures during early elongation phase. Scale bar, 2μm.