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. 2018 May 23;13(5):e0198014. doi: 10.1371/journal.pone.0198014

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© 2018 David et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 5 — Methicillin-induced filaments expressing FtsZ-Ve (NZ3900 [pGIBLD031]) (A) or Ve-PBP2b (NZ3900 [pGIBLD031]) (B) were transferred to methicillin-free agar pads to study their localization dynamics by time-lapse microscopy (phase contrast (PC) and fluorescence) during filament reversion (see also S5–S10 Movies). Localization of the protein at the constricting septum and future division sites is highlighted with red and orange arrows, respectively. Only the early steps of filament reversion are highlighted. Scale bars, 2 μm. (C) Schematic diagrams summarizing localization patterns of FtsZ-Ve (green) and Ve-PBP2b (yellow).