Table 1. Bacterial strains and plasmids.
| Strain or plasmid | Characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| L. lactis | ||
| NZ3900 | MG1363 derivative containing the nisR and nisK genes stably integrated at the pepN locus | [22] |
| BLD001 | NZ3900 pbp1a::pCM1837 | This study |
| BLD002 | NZ3900 pbp1b::pED 2045 | This study |
| BLD003 | NZ3900 pbp2a::p2092 | This study |
| BLD004 | NZ3900 pbp2b::p2081 | This study |
| BLD006 | NZ3900 dacA::pGIBLD006 | [12] |
| Plasmids | ||
| pCM1837 | Emr a; suicide plasmid pRV300 containing a ~200-pb disruption cassette of pbp1a | [23] |
| pED2045 | Emr; suicide plasmid pRV300 containing a 768-pb disruption cassette of pbp1b | Domakova E. and Kulakauskas S., INRA b |
| p2092 | Emr; suicide plasmid pRV300 containing a 481-pb disruption cassette of pbp2a | Budin-Verneuil A. and Maguin E., INRA b |
| p2081 | Emr; suicide plasmid pRV300 containing a 1073-pb disruption cassette of pbp2b | Budin-Verneuil A. and Maguin E., INRA b |
| pGIBLD006 | Emr; suicide plasmid pUC18ery containing a 555-pb disruption cassette of dacA | [12] |
| pNZ8048 | Cmr a; multicopy plasmid containing the inducible nisA promoter (PnisA), designed for translational fusions | [24] |
| pGIBLD027 | Cmr; pNZ8048 derivative containing pbp2b under the control of PnisA | This study |
| pGIBLD0271 | Cmr; pNZ8048 derivative containing an inactive variant of pbp2b (catalytic residue Ser414 of PBP2b mutated in Ala) under the control of PnisA | This study |
| pGIBLD008 | Cmr; pNZ8048 derivative containing the Venus encoding gene. | [25] |
| pGIBLD031 | Cmr; pGIBLD008 derivative expressing a FtsZ-Venus fusion protein (Venus in C-terminus of FtsZ). | This study |
| pGIBLD041 | Cmr; pGIBLD008 derivative expressing a Venus-PBP2b fusion protein (Venus in N-terminus of PBP2b). | This study |
a Cmr and Emr indicate resistance to chloramphenicol and erythromycin, respectively.
b INRA laboratory collection