Fig. 2. NIRS predictions of ZIKV infection in heads/thoraces and abdomens of A. aegypti mosquitoes on a blind data set.

(A) Predicted infections at 4, 7, and 10 dpi in heads/thoraces for the subset of cohort 1 (n = 155) that were excluded from the model and all cohort 2 samples (n = 412). (B) Predictions for abdomens from cohort 2 samples (n = 412). Each circle represents an individual mosquito; infection status confirmed by RT-qPCR is indicated by solid (infected) or empty (uninfected) circles. Red lines indicate the mean prediction value for each group. The vertical axis indicates infection status as predicted by NIRS, with the dotted line indicating the classification cutoff point. Infected mosquitoes shown below the dotted line and uninfected mosquitoes shown above the dotted line were falsely predicted.