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. 2018 May 23;4(5):eaap8957. doi: 10.1126/sciadv.aap8957

Fig. 1. AAV7-MANF injection after ischemia results in enhanced behavioral recovery.

Fig. 1

(A) The rats underwent dMCAo surgery, and on day 1, they were divided into two groups, balanced according to the severity of their neurological deficit assessed by the EBST and BNST. On day 2, the animals received intracerebral injections of either AAV7-MANF or AAV7-GFP, and their behavioral recovery was evaluated on days 7 and 14. (B) Expression patterns of hMANF 2 weeks after intracerebral AAV7-MANF injection. Coronal sections of the lesioned cerebral hemisphere were immunostained using anti-hMANF antibody. Sections from positions close to injection sites (at approximately A/P +1.6 and A/P −0.4) are presented. The core of the ischemic lesion is outlined. Black and gray arrows mark the dorsal and lateral peri-infarct cortex, respectively, where the deeper layers are AAV7-transduced. (C) Close-up of hMANF expression in the peri-infarct region encompassing cortex (ctx), striatum (str), and external capsule (ec). Scale bar, 100 μm. (D and E) The behavior of rats was assessed with BNST (D) and the EBST (E) on days 7 and 14 after the dMCAo surgery (n = 9 in both groups). *P ≤ 0.003 by Mann-Whitney U test. (F) Forepaw use bias of AAV7-GFP– and AAV7-hMANF–injected rats as determined using the cylinder test. According to a two-way analysis of variance (ANOVA) [F(1,32) = 2.2056, P = 0.1613], the differences in right forepaw use were not statistically significant. Average ± SEM is shown.