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. 2018 May 23;4(5):eaap8957. doi: 10.1126/sciadv.aap8957

Fig. 4. AAV7-MANF treatment increases the number of phagocytic immune cells in the subcortical regions.

Fig. 4

(A) qPCR analysis of Emr1, C3, Cfp, and Cd8a mRNA levels in the peri-infarct cortex at day 4 after ischemic injury (n = 4 to 5). ***P ≤ 0.0004 by Tukey’s multiple comparisons post hoc test, following one-way ANOVA (P < 0.0001). (B and C) Density of CD68+ objects in the corpus callosum/external capsule underlying the infarct at day 4 after ischemic injury. ***P = 0.0001. (D) Density of CD68+ objects in the dorsal striatum. **P = 0.0099. (E and F) Density of CD68+ objects in the lateral peri-infarct cortex. P = 0.7504. (G and H) Density of Arg1+ objects in the corpus callosum/external capsule. **P = 0.0053. (I) Density of Arg1+ objects in the dorsal striatum. **P = 0.006. (C to I) The individual points on graphs present the average of two coronal sections, taken from positions A/P +0.1 and −0.7. AAV7-GFP (n = 6) or AAV7-hMANF (n = 7). Representative image is shown for each area. Scale bars, 100 μm. (J) qPCR analysis of Manf mRNA levels in the injured and contralateral (contral.) cortex of Manffl/fl and NestinCre/+::Manffl/fl mice at 2 days after focal ischemic injury (n = 4 to 6). **P < 0.002 by Tukey’s multiple comparisons post hoc test, following one-way ANOVA (P < 0.0001). (K) Infarct volumes (averaged with SEM) in Manffl/fl and NestinCre/+::Manffl/fl mice sacrificed 2 days after a permanent MCAo. Lesion volumes were quantified from triphenyl tetrazolium chloride–stained brain sections. Student’s t test was used for the analysis of statistical significance. Average ± SEM is shown.