Figure 2. Discovery phase flow-chart.

^§Supplementary Table 2 displays a detailed description of the pooled groups. ^*Mann-Whitney U test. QC = Quality control. ^¶Too high expressed in multiplex vs uniplex; appears to detect additional transcripts. ^¥Poor amplification and too low expressed for a reliable analysis. ±Too low expressed for a reliable analysis. ^**Poor amplification curves and poor efficiency in multiplex.