Extended Data Table 1.

Wnt pathway gene expression levels in different cell populations as assessed by RNAseq (mean of 2–3 samples each in FPKM)

Wnt pathway
Gene name	Foxl1+ Expression level mean (FPKM)	Mes Expression level mean (FPKM)	Lgr5 Expression level mean (FPKM)	Diff Expression level mean (FPKM)
Wnt2	0.9742	0	0.1736	0
Wnt2b	9.5831	0.1205	0.1559	0.1191
Wnt3	0.3438	0	1.0915	0.0909
Wnt4	1.7951	0	0.0102	0.9198
Wnt5a	4.9932	0	0.1219	0.0786
Wnt5b	0.8935	0	0.5514	0.3542
Wnt6	0.0866	0	0.0824	0.3045
Wnt9a	0.5072	0	0.0505	0.0553
Wisp1	2.4082	0	0.0286	0
Rspo2	0.4112	0	0	0
Rspo3	2.1902	13.3906	0.0141	0
Lgr4	7.5384	0	48.6203	9.8020
Lgr5	1.9220	0	19.3406	0.0408
Lgr6	0.2264	0	0	0
Znrf1	4.2325	0	3.6232	0.8523
Znrf2	2.2594	5.0671	12.7731	7.7214
Znrf3	0.9130	0.1328	1.9908	0.1333
Dkk2	1.8779	0	0	0
Dkk3	36.5673	22.1995	0.0017	0.0077
Wif1	1.2579	0	0.0125	0.0844
Sfrp1	47.2422	0.6198	0.0722	0.0034
Sfrp4	0.7134	0	0.0096	0
Fzd2	2.50329	0	1.9856	0.0100
Fdz4	4.5451	0	0.2025	0.9071
Fzd6	0.5785	0	2.7795	0.2181
Fzd7	1.1146	0	16.7951	2.5999
Fzd8	0.6245	0	0.7510	0.3280
Lrp1	11.4018	0.0144	6.4567	12.5752
Lrp6	6.0929	0.0013	9.5234	4.2674
Tcf4	15.0228	8.338	6.0325	0.9127
Tcf15	1.71113	0	0.0090	0
Tcf19	1.456	8.768	13.6231	0.7681
Tcf21	19.6602	0.0065	0	0
Tcf7l1	1.8914	0	0.1070	0.0623