Figure 2.

G-Rg5 and G-Rk1 inhibited interaction between Annexin A2 and NF-κB p50 subunit and their nuclear co-localization. (A) Immunoprecipitation was performed with whole-cell lysate of HepG2 cells under treatment with G-Rg5 (6 μmol/L), G-Rk1 (6 μmol/L), etoposide (25 μg/mL) and PMA (100 ng/mL), and the interaction was analyzed by an immunoblot. (B) Immunoprecipitation was performed with prokaryotic cells-expressed Annexin A2 and NF-κB p50 subunit under treatment with G-Rg5 (6 μmol/L) and G-Rk1 (6 μmol/L), and the interaction was analyzed by an immunoblot. (C) The subcellular distribution of Annexin A2 and NF-κB p50 subunit was examined by immunofluorescence under treatment with G-Rg5 (6 μmol/L), G-Rk1 (6 μmol/L), etoposide (25 μg/mL) and PMA (100 ng/mL), and DAPI showed the nuclear region