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. 2018 Mar 9;37(21):2873–2889. doi: 10.1038/s41388-018-0183-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — MiR-23a is highly expressed in NPC-exo. a Scheme of exosome isolation by differential ultracentrifugation. b Representative electron microscopy image of NPC-exo. c Nanoparticle tracking analysis displayed the size distribution of exosomes isolated from NPC. d Western blot analysis of exosomal markers. CNE2 cell line was used as controls for exosomes characterized. Flotillin-1 was used as a loading control. e Uptake of exosomes in HUVECs by confocal microscopy. Blue: Hoechst staining; green: PKH67-labeled exosomes. f, g qRT-PCR of miRNA level in exosomes isolated from serum (grouping based on the clinical features of patients at the blood-drawing time) or NPC cells. T test. h qRT-PCR of miRNA expression in exosomes isolated from miR-23a-treated CM. One-way ANOVA