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. 2018 Mar 9;37(21):2873–2889. doi: 10.1038/s41388-018-0183-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 7 — MiR-23a directly targets TSGA10. a Schematic of predicted miR-23a binding sequence in TSGA10-3′-UTR. b Representative IHC images of TSGA10 in tissues collected from graph-depicted groups. TSGA10 staining was mainly localized in the cytoplasm of cells. c Overexpression of miR-23a reduced TSGA10-3′-UTR luciferase activity in vitro but not mutated TSGA10-3′-UTR luciferase activity. Student’s t-test. d–g TSGA10 immunoblotting in HUVECs treated as indicated. The results were analyzed with ImageJ software. One-way ANOVA. h, i Interference efficiency was detected by western blot. One-way ANOVA. j, k Transwell migration assays were performed to measure cell migration. One-way ANOVA. l, m HUVECs treated as indicated and analyzed by western blot. Student’s t-test. n, o Transwell migration assays were performed to measure cell migration. One-way ANOVA. p–r HUVECs treated as indicated were analyzed by western blot. One-way ANOVA. s Transwell migration assays were performed to measure cell migration. One-way ANOVA