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. 2018 May 23;4:11. doi: 10.1038/s41526-018-0045-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — Morphological evaluation of myogenesis under microgravity conditions. a Schematic representation of experimental time course is shown. L6 cells were cultured with growth medium for 2 days, and then replaced with differentiation medium. Cells were then cultured under normal 1 G or 10^-3G conditions for 0, 12 h, 1, 3, 5, or 7 days, and observed by taking pictures, and the maximum transverse diameters of myotube cells (myotube thickness) were measured. b Representative microscopic images during myogenesis are shown. White arrow heads: myotube cell; white H-shaped bars: examples of the maximum transverse diameter of myotube cells; white scale bar: 300 μm. c The maximum transverse diameters of myotube cells were measured by image processing software ImageJ, and the mean value of 10 fields was calculated. Columns show the mean of three independent experiments; bars, SD. P values calculated with Student’s t test. (**P < 0.01) (n = 3)