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. 2018 May 23;4:11. doi: 10.1038/s41526-018-0045-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — Gene expression analyses of myogenesis-related genes under microgravity conditions. For expression analysis, L6 cells were cultured under normal 1 G or 10^-3G conditions for 0, 12 h, 1, 3, 5, or 7 days. Total RNA was extracted from cell pellets, and expression levels of myogenesis-relating genes were evaluated by real-time RT-PCR. Three independent measurements were averaged, and relative gene expression levels were calculated as a ratio against Actb expression for each experiment. Columns show the mean of three independent experiments; bars, SD. P values calculated with Student’s t test. (*P < 0.05, **P < 0.01) (n = 3)