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. 2018 May 23;4:61. doi: 10.1038/s41420-018-0062-5

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a 293T cells were transfected with His-Src, caspase-9, or both. Cells were harvested 24 h after transfection. The expression level of several apoptotic proteins was detected by western blotting. b After transfection, 293T cell lysates were collected. Caspase-9 activity was measured using the Caspase-9 Colorimetric Assay kit. Data are shown as means ± S.D. of triplicate measurements. The asterisks indicate a significantly higher caspase-9 activity (*p < 0.05)