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. 2018 May 23;9(6):610. doi: 10.1038/s41419-018-0677-8

Fig. 4. BIRC6 is regulated by miR-181a directly.

Fig. 4

a The schematic of the luciferase reporter containing the binding sites for miR-181a at the 3′-untranslated regions (3′-UTR) of BIRC6 from different species. The sequences of BIRC6 3′-UTR mutants used for luciferase reporter assay are presented. b, c Luciferase reporter constructs containing 3′-UTR (b) or mutant one 3′-UTR (3′-UTR mutant 1) or mutant double 3′-UTR (3′-UTR double mutant) (c) of BIRC6 was co-transfected with miRNA negative control (NC-m), miR-181a mimics (miR-181a-m), miRNA inhibitor negative control (NC-i), or miR-181a inhibitor (miR-181a-i) in mesangial cells and the luciferase activity were determined. d, e Mesangial cells were transfected with miR-181a mimics or miR-181a inhibitor for 48 h. qRT-PCR and western blotting were performed to examine endogenous BIRC6 mRNA (d) and protein (e) expression. **p < 0.01 vs. miRNA negative control or miRNA inhibitor negative control, n = 6