Fig. 5. Knockdown of miR-181a alleviated kidney injury induced by 5-FU.

a Experimental design to examine the effect of miR-181a inhibition on 5-FU-induced kidney injury. Mice were injected with adeno-associated virus encoding miR-181a tough decoy (AAV-TuD-181a) or AAV-TuD-GFP for 14 days and then treated with 5-FU (200 mg/kg body weight) for 4 days. b On the 18th day, mice were sacrificed, and the miR-181a expression in kidneys was determined. c–e Serum blood urea nitrogen (BUN) (c), creatinine (d), and LDH (e) concentration were tested by biochemical assay. **p < 0.01 vs. control; ^##p < 0.01 vs. 5-FU, n = 5–10. f Histological examination of renal cortex section was performed using hematoxylin & eosin (H&E) staining. n = 5