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. 2018 May 23;9(6):610. doi: 10.1038/s41419-018-0677-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b Immunohistochemical staining of Ly-6G (a) and CD68 (b) in kidney tissues of mice treated with TuD-GFP or TuD-181a under basal level or after 5-FU administration. c, d Quantification of Ly-6G-positive neutrophils (c) and CD68-positive macrophages (d) areas using Image-Pro 5.0 software. e The myeloperoxidase (MPO) activity in kidney tissues was determined. f–h The mRNA expressions of IL-1β (f), IL-6 (g), and TNF-α (h) were determined by qRT-PCR, respectively. **p < 0.01 vs. control; ^##p < 0.01 vs. 5-FU, n = 4–8