Fig. 2.

In vitro screening of a focused library identifies TSL2-interacting small molecules. a Selected scaffolds for the building of a 304-compound, focused RNA-binding chemical library. b Ranking of positive hits (100 μM) from the primary TSL2-interacting screening, with a cut-off value of 20% fluorescence displacement (FD) relative to DMSO (1%) controls (n = 4). Hits NCI377363, PK4C9, BJGF466 and 288D are highlighted. c EC50 binding curves and values of hits NCI377363, PK4C9 and BJGF466 to TSL2 (n = 8; two plates with four replicates each). An EC50 value for 288D could not be calculated due to poor solubility. d Chemical structures of NCI377363, PK4C9, BJGF466 and 288D. Graphs represent mean values ± SEM