Fig. 3.
Demethylation and gene expression. a Percentage of DMRs (WGBS) located in genes (see Methods) that are upregulated with age (n = 8500) compared to randomly selected DNA segments (p value <10−27, z-test of proportions). b Box plots of relative expression (normalized fragments per kilobase million (FPKM)) in WT and Tet2/Tet3-deficient hepatocytes for 1212 genes upregulated after birth that contain intragenic DMRs. The DKO was induced at either 1 day or 15 weeks. Similar results were obtained using WT animals injected with AAV8-cre. c Expression of 115 genes that undergo demethylation (RRBS) and that are upregulated between 3 and 15 weeks. The DKO was induced at 3 weeks. d Expression of 47 genes that undergo demethylation (RRBS) and that are upregulated between 1 and 3 weeks. The DKO was induced at 3 weeks. In the DKO, the pattern of expression at 15 weeks was very similar to that of WT animals at 3 weeks