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. 2018 May 23;8:8053. doi: 10.1038/s41598-018-26442-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Phospho-SMAD2 (Ser465/467) (P-SMAD2) is detected in the nuclei of ECs of normal choroidal blood vessels but not in ECs of the CNVMs of AMD patients. Fixed paraffin-embedded CNVM sections from 2 AMD patients and human eye sections containing normal choroidal blood vessels were analyzed by immunofluorescence using phospho-SMAD2 (green) and CD34 (red) antibodies. Arrowheads indicate P-SMAD2 positive nuclei. Arrows and asterisks indicate the retinal pigment epithelium and red blood cells, respectively, which exhibits autofluorescence. Scale bars, 14 μm.