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. 2018 May 23;8:8044. doi: 10.1038/s41598-018-26479-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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mTORC1 blockade impairs auditory and visual imprinting. (a) Chickens were trained for two hours and injected either with rapamycin (N = 11) or vehicle (N = 10) prior to training. Visual and auditory imprinting were separately tested the day after training. (b) Rapamycin (N = 7) strongly reduced phosphorylation of S6 within MNM (left) and IMM (right) compared to vehicle-injected chickens (N = 7). (c) Vehicle- (N = 11), but not rapamycin-injected (N = 10) chickens showed auditory (left) and visual (right) imprinting the day after training. Box plots show mean and interquartile. Error bars show 10–90 percentile, *indicates p < 0.05 from two-sample t-test. Number of animals used for each group is inside parenthesis.(Illustrations by Michael Beckert).