Table 3.
Genes significantly inhibited in MCF-7 cells after 6 h. of 13.5 µM anacardic acid (AnAc) treatment.
| Gene | Control | AnAc | P value | Description | Top 3 GO terms |
|---|---|---|---|---|---|
| RBMS1 | 35.69 | 15.14 | 5.00E-05 | RNA Binding Motif Single Stranded Interacting Protein | GO:0006396: RNA processing; GO:0006260: DNA replication; GO:0003697: single-stranded DNA binding |
| SCD | 400.61 | 234.01 | 0.001 | stearoyl-CoA desaturase | GO:0004768: stearoyl-CoA 9-desaturase activity;GO:0006633: fatty acid biosynthetic process;GO:0005506: iron ion binding |
| RNU4-2 | 104.59 | 27.41 | 0.0016 | U4 Small Nuclear 2 | |
| TSPAN33 | 104.59 | 27.41 | 0.0034 | Tetraspanin 33 | GO:0016021; integral to membrane |
| STIM1 | 25.05 | 10.56 | 0.0038 | Stromal Interaction Molecule 1 | GO:0006812: cation transport; GO:0043234: protein complex; GO:0005515: protein binding |
| GATA6-AS1 | 1.19 | 0.19 | 0.0047 | GATA6 Antisense RNA 1 (Head To Head) | |
| TGM2 | 3.82 | 2.27 | 0.0069 | Transglutaminase 2 | GO:0043277: apoptotic cell clearance; GO:0018149: peptide cross-linking; GO:0060662: salivary gland cavitation |
| CD22 | 1.04 | 0.57 | 0.0161 | Sialic Acid Binding Ig-Like Lectin 2 | GO:0009897:external side of plasma membrane; GO:0005887: integral to plasma membrane; GO:0005515:protein binding |
| RN7SL389P | 1.15 | 0.00 | 0.02285 | RNA, 7SL, Cytoplasmic 389, Pseudogene | |
| CDIP1 | 1.25 | 0.61 | 0.0229 | Cell Death-Inducing P53 Target 1 | GO:0042771: intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator; GO:0033209: tumor necrosis factor-mediated signaling pathway; GO:0006915:apoptotic process |
| INSIG1 | 86.16 | 64.07 | 0.0285 | Insulin Induced Gene 1 | GO:1901303: negative regulation of cargo loading into COPII-coated vesicle; GO:0032937: SREBP-SCAP-Insig complex GO:0032933: SREBP signaling pathway |
| LAMC2 | 5.68 | 3.17 | 0.03285 | Laminin Subunit Gamma 2 | GO:0005610: laminin-5 complex; GO:0034329: cell junction assembly; GO:0031581: hemidesmosome assembly |
| SAMD9 | 2.16 | 1.44 | 0.0353 | SAM Domain-Containing Protein 9 | GO:0043231: intracellular membrane-bounded organelle; GO:0005737: cytoplasm; GO:0005515: protein binding |
| ZNF462 | 1.35 | 0.67 | 0.0413 | Zinc Finger Protein 462 | GO:0006325: chromatin organization; GO:0043392: negative regulation of DNA binding; GO:0003677: DNA binding |
| MIR22HG | 12.49 | 5.52 | 0.0421 | MIR22 Host Gene | |
| MALL | 15.93 | 10.98 | 0.04315 | Mal, T-Cell Differentiation Protein-L; Protein BENE | GO:0030136: clathrin-coated vesicle; GO:0016023: cytoplasmic membrane-bounded vesicle; GO:0042632: cholesterol homeostasis |
| RNU5B-1 | 76.15 | 25.32 | 0.0453 | U5B Small Nuclear 1 | |
| RNU4-1 | 57.57 | 26.33 | 0.0467 | U4 Small Nuclear 1 | |
| EGR1 | 17.13 | 13.03 | 0.0474 | Early Growth Response 1 | GO:0072303: positive regulation of glomerular metanephric mesangial cell proliferation GO:0072110: glomerular mesangial cell proliferation; GO:0071873: response to norepinephrine |
Genes are arranged from the most to least statistical significance. Values are FKPM. All values are significantly different, P < 0.05. The GO terms are listed in the order provided by DEG analysis and subsequent network analysis in MetaCore Gene Ontology (GO) algorithm to characterize the biological pathways altered by AnAc.