Fig. 1. Metformin attenuates the migratory and invasive capacities of osteosarcoma cells.

a CCK8 assays showed that metformin did not appreciably affect the vitality of MG63,U2OS or 143B cells at concentrations of 2, 5 or 10 mM (left). A similar result was detected in FCM experiments (right). b Metformin reduces the migration of osteosarcoma cells in wound-healing assays. Migration rates were calculated by the healing area/wound area after 24 h. c Metformin significantly suppresses the migration (left) and invasion (right) of osteosarcoma cells with transwell analysis. (*P < 0.05, **P < 0.01). d Cytoskeletal assay of 143B and U2OS cells was visualized by confocal microscopy. Representative images were shown. Cell nuclei were stained with DAPI. Scale bar represents 25 μm or 10 μm