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. 2018 May 23;9(6):611. doi: 10.1038/s41419-018-0620-z

Fig. 6. LCMR1 and Atg12 are targets of miR-570-3p and initiators of metformin-induced metastasis inhibition and autophagy reduction.

Fig. 6

a The LCMR1 and Atg12 3′UTR region containing the wild type or mutant binding site for miR-570-3p. b Dual-luciferase assays were performed in 143B cells after co-transfection with wild-type or mutant LCMR1 and Atg12 3′-UTR plasmids and with NC or miR-570-3p mimics. c Western blot and FCM demonstrated the significant downregulation of LCMR1 and Atg12 in miR-570-3p-overexpressing cells compared with NC cells. d Upregulation of LCMR1 and Atg12 in miR-570-3p knockdown cells. e Metformin inhibits LCMR1 and Atg12 in a time and dose-dependent manner. f Knockdown of miR-570-3p blocked the metformin-induced inactivation of LCMR1 and Atg12 in 143B and U2OS cells. g The expression of LCMR1 and Atg12 in metastatic and primary non-metastatic osteosarcoma tissues