Fig. 7. Metformin up-regulates the expression of miR-570-3p and inhibits metastasis in vivo.

a Observation of tumors formed by 143B cells (left). qRT-PCR analyses of miR-570-3p, LCMR1 and Atg12 levels in xenografts (right) (mean ± SD, n = 3). **P < 0.01 compared with tumors treated with DMSO. b Immunohistochemistry analyses of LCMR1 and Atg12 levels. c Western blot analyses of LCMR1 and Atg12. d Number of metastatic nodules on the surface of the lungs of mice injected with DMSO or metformin is presented. Representative images and H&E staining of lungs on day 70 after the mice were injected with 143B cells (n = 6 per group). e Number of metastatic nodules on the surface of the lungs of mice formed by stable anti-miR-570-3p or anti-con 143B cells is presented