Figure 7.

Inhibition of CHK2 activity reduces JEV replication in human BE(2)C neuroblastoma cells. BE(2)C cells were infected with JEV at MOI 0.1, 1 or 5 in the absence (DMSO solvent control) or presence of 25 or 50 μM CHK2 inhibitor II as indicated. (A) Immunoblotting analysis of cell lysates at 24 h with antibodies against T68-phosphorylated CHK2, CHK2 and actin. BE(2)C cells were treated with DMSO or CHK2 inhibitor II (50 μM), followed by JEV infection at MOIs of 0.1 (B) or 5 (C) for the indicated times. Culture supernatants were collected for virus titration by plaque-forming assays. Data are mean ± SD virus titers (n = 3). ^*p < 0.05; ^**p < 0.01 ^***p < 0.005 by two-tailed Student t-test.