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. 2018 May 17;8:142. doi: 10.3389/fcimb.2018.00142

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Copyright © 2018 Chan, Liao and Lin.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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JEV infection induces cell cycle arrest at G1 phase. U87 (A), A549 (B), and BE(2)C (C) cells were mock-infected, JEV (MOI 5) infected, or X-ray (8 Gy) treated. At 24 h post-treatment, DNA content was determined by PI staining and analyzed by flow cytometry for proportion of cells in G1, S, and G2 phases.