Fig. 2.

Endocannabinoid receptor 2 (CB2R) protein expression in the maternal and fetal livers of high-fat diet (HFD; n = 11) and control (CTR; n = 10) animals. A–D: representative images of fetal livers from male fetuses (MF) in the CTR (n = 3) and HFD (n = 6) diet groups. E–H: the fetal livers from female fetuses (FF) in the CTR (n = 4) and HFD (n = 4) groups. I–L: maternal livers of dams with MF in the CTR (n = 3) and HFD (n = 5) groups. M–P: maternal liver sections from dams carrying FF in the CTR (n = 5) and HFD (n = 4) groups. Q–T: quantitative analyses. Blue arrows point to bile ducts, green arrows point to arteries, pink arrows point to central veins (CV), and yellow asterisks indicate the portal vein. Negative controls (omission of primary antibodies) are shown in A–P, insets (top right). Scale bars, 20 μm. The data are presented as means ± SE.