Table 2.

AEA and 2-AG concentrations by diet and fetal sex

	Female Fetuses		Male Fetuses		P Value
	CTR	HFD	CTR	HFD	Diet	Sex	Diet × sex
Fetus
AEA, nM/l	0.71 ± 0.17 (n = 4)	0.89 ± 0.22 (n = 5)	1.10 ± 0.13 (n = 5)	0.65 ± 0.05 (n = 6)	0.21	0.53	0.06
2-AG, nM/l	53.99 ± 12.82 (n = 4)	22.94 ± 3.22 (n = 5)	59.40 ± 26.38 (n = 5)	22.79 ± 3.66 (n = 6)	0.01	0.51	0.58
Mother
AEA, nM/l	1.82 ± 0.19 (n = 3)	1.81 ± 0.17 (n = 5)	1.46 ± 0.28 (n = 5)	1.83 ± 0.17 (n = 6)	0.6	0.44	0.4
2-AG, nM/l	86.26 ± 11.86 (n = 3)	109.96 ± 29.08 (n = 5)	82.21 ± 11.76 (n = 5)	194.27 ± 30.61 (n = 6)	0.05	0.25	0.17
Maternal/fetal ratio
AEA	3.58 ± 0.84 (n = 3)	2.37 ± 0.42 (n = 5)	1.39 ± 0.30 (n = 5)	2.91 ± 0.39 (n = 6)	0.5	0.16	0.03
2-AG	2.27 ± 0.73 (n = 3)	4.84 ± 1.00 (n = 5)	3.89 ± 2.30 (n = 5)	9.36 ± 1.64 (n = 6)	0.02	0.28	0.53

Values are means ± SE. AEA, anandamide; 2-AG, 2-arachidonoyl glycerol; CTR, control; HFD, high-fat diet.