Fig. 2.

Low-dose DHT caused insulin resistance in the liver and WAT but not in the pituitaries and ovaries of fasted mice. At 3 mo post-insertion, control and DHT mice were fasted for 16 h and then injected with saline or 2 U/kg insulin. After 10 min, livers, WAT, pituitaries, and ovaries were harvested and subjected to Western blot analysis A–D) and multiplex Luminex assay analysis E–H) of p-AKT S473 and AKT. Livers (I) and WAT (J) were harvested from fed control and DHT mice and processed for Western blot analysis using antibodies against p-AKT, AKT, and/or actin; n = 4–7/group. Unpaired 2-tailed t-tests were used comparing control with the tested groups and insulin only to the insulin plus DHT group. Different letters indicate a statistically significant difference. Same letters indicate no statistically significant difference. *P < 0.05.