Fig. 4.

Low-dose DHT did not result in insulin resistance in skeletal muscles. Skeletal muscles extracted from fed control and DHT mice (A) and from control and DHT mice that were fasted for 16 h then injected with saline or 2 U/kg insulin (B) were harvested and subjected to Western blot analysis for p-AKT S473 and AKT. Basal (C) and insulin-stimulated (D) ex vivo 3H-2-deoxy-glucose transport assays were performed in extracted skeletal muscles. E: skeletal muscles of control and DHT mice were harvested and processed for qRT-PCR analysis using TRIzol for RNA isolation. F: skeletal muscles from control and DHT mice were harvested in the fed state and processed for Western blot analysis after cell lysis. See Table 1 for a list of the abbreviations and functions of qRT-PCR primers and Table 2 for information on antibodies used for Western blotting; n = 10/group for qRT-PCR and n = 4/group for Western blotting; *P < 0.05, **P < 0.01, ***P < 0.001. Bas, basal; Ins, insulin; C, control; D, DHT, dihydrotestosterone; n = 4–7/group; different letters indicate significance.