Fig. 6.

DHT mice displayed impaired glucose tolerance, impaired insulin sensitivity, and reproductive dysfunction seen as reduced corpora lutea, increased atretic follicles, and acyclity. A and B: control and low-dose DHT mice fasted for 16 h received intraperitoneal injections of 2 g/kg body wt glucose (GTT) or 0.3 U/kg body wt insulin (ITT; Lilly, Indianapolis, IN), and tail blood was obtained to determine blood glucose at time points between 0 and 120 min using a One Touch Ultra glucometer and One Touch Ultra test strips (Life Scan, Milpitas, CA). C: the estrus cycle was tested by obtaining vaginal smears for 14 consecutive days and analyzing the cytology of the smears. D: at 3 mo post-insertion of DHT, ovaries of control and DHT mice were obtained, sectioned at 5 mm, and morphological analyses were performed. Number of atretic follicles (AF; E) and corpora lutea (CL; F) were determined as previously described (25); n = 6–8/group. *P< 0.05, **P < 0.01, ***P < 0.001.