Fig. 1.

Generation of Aqp2^ECE/+ mice. Aqp, aquaporin; ECE, cassette expressing estrogen receptor (ER)^T2CreER^T2. A: schematic illustration of targeting strategy for the Aqp2^ECE knock-in allele. B and C: Southern blotting analyses of embryonic stem (ES) cell clones confirming the targeted Aqp2^ECE knock-in allele. D: PCR-based genotyping of chimeras showing male 5022 with almost 100% contribution of ES cells, as evidenced by the presence of the ES cell-derived WT allele coupled with barely detectable host blastocyst mutant allele of nicotinamide nucleotide transhydrogenase gene (Nnt). E: PCR-based genotyping showing that male 5022 had a high rate in germline transmission of Aqp2^ECE allele to offspring.