Fig. 2.
Analyses of Aqp2 expression. Total RNAs and proteins were isolated from kidneys of uninduced Aqp2+/+ and Aqp2ECE/+ kidneys with free water access (n = 3 mice/genotype, A–C) and under water deprivation for 24 h (n = 4 mice/genotype, D–F). Each RNA sample was analyzed by real-time RT-qPCR in triplicates, using GAPDH as internal control. For Western blotting analyses, rabbit Aqp2 (sc-28629) was used. The 35- to 50-kDa band corresponding to glycosylated Aqp2 was quantified, normalized to β-actin, and presented in C and F, respectively. In all case, relative Aqp2 mRNA and protein levels in WT were set to 1. *P < 0.05 vs. WT.