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Aqp2ECE possesses absolute no leaky activity. A–C: immunofluorescence staining for Aqp2 (green) and red fluorescent protein (RFP; red) showing no RFP expression in whole kidney of an oil-injected adult ECE/+RFP/RFP mouse. Cells indicated by arrowheads were magnified in the insets. Scale bar, 50 μm, 8.3 μm for inset.
