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Aqp2ECE is inactive in intercalated cells marked by V-ATPase subunits B1 and B2 (B1B2). A–C: immunofluorescence staining for Aqp2 (blue) and B1B2 (green) to mark intercalated cells and RFP (red) showing no RFP coexpression with B1B2 in whole kidney of adult ECE/+RFP/RFP mice treated with a single dose of tamoxifen (2 mg) for 24 h. Cells indicated by arrowheads are magnified in the insets. Scale bar, 50 μm, 8.3 μm for inset.
