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Aqp2ECE is principal-cell specific. A–C: immunofluorescence staining for Aqp3 to mark principal cells (blue) and RFP (red) showing that all RFP-expressing cells also expressed Aqp3 in whole kidney of adult ECE/+RFP/RFP mice treated with a single dose of tamoxifen (2 mg) for 24 h. Cells indicated by arrowheads are magnified in the insets. Scale bar, 50 μm, 8.3 μm for inset.
