Table 1.
Primer list
| Primers | Note | Sequence (5′ to 3′) |
|---|---|---|
| WZ1026 | Aqp2-COM forward | AAGTGCCCACAGTCTAGCCTCT |
| WZ1028 | Aqp2-WT reverse | GGAGAACGCTATGGACCGGAGT |
| WZ1094 | Aqp2 forward | ATGTGGGAACTCCGGTCCATA |
| WZ1095 | Aqp2 reverse | ACGGCAATCTGGAGCACAG |
| WZ1106 | GAPDH forward | AGGTCGGTGTGAACGGATTTG |
| WZ1107 | GAPDH reverse | TGTAGACCATGTAGTTGAGGTCA |
| WZ1631 | Aqp2-ECE reverse | TCGCCGCTCCCGATTCGCAG |
| WZ1494 | 5′ arm reverse | AAGGTTGGCAGCTCTCATGTCTCCAGCCATGCTGCTCGGCCTTCTGAGCGCTGGCCAGTGGTCT |
| WZ1495 | 5′ arm ECE junction forward | AGACCACTGGCCAGCGCTCAGAAGGCCGAGCAGCATGGCTGGAGACATGAGAGCTGCCAACCTT |
| WZ1496 | Cre reverse | TACACCAGAGACGGAAATCCATCGCTCG |
| WZ1499 | 3′ arm forward | ATCTGTGTATACTGGGAACTCCGGTCCATAG |
| WZ1500 | 3′ arm reverse | CAGACTACATGTTAGGTCATACTGATACAACAGGATGC |
| WZ1502 | 5′ arm forward | ATCTGTGAATTCCTGGCTCCCTAAACCTCCTATG |
| WZ1643 | Nnt-COM forward | GTAGGGCCAACTGTTTCTGCATGA |
| WZ1644 | Nnt-WT reverse | GGGCATAGGAAGCAAATACCAAGTTG |
| WZ1645 | Nnt-MT reverse | GTGGAATTCCGCTGAGAGAACTCTT |
| TMF241 | 5′ probe forward | CCGCACCGTGGAAATGTGGATG |
| TMF242 | 5′ probe reverse | TCAACCATAGCACACCCAGAAAGGGT |
| TMF571 | 3′ probe forward | TCGGTTCCCAGTGCAGGGTAGCTAG |
| TMF572 | 3′ probe reverse | TCCAGGACTCCCTTGCGAAACCCGA |
Aqp2, aquaporin 2; ECE, cassette expressing estrogen receptor (ER)T2CreERT2; Nnt, nicotinamide nucleotide transhydrogenase.