Fig. 3.
Immunoblot metabolic enzyme content in subsarcolemmal mitochondria (SSM). Compared with the time control, ischemia-reperfusion (I/R) markedly decreased the content of the full-length pyruvate dehydrogenase (PDH) α1-subunit and increased its cleaved products. A: amobarbital treatment maintained the PDH content in SSM after ischemia-reperfusion. I/R also decreased the contents of enoyl coenzyme A hydratase, short chain, 1 mitochondrial (ECSH1) and malate dehydrogenase 2 (MDH2) compared with the time control. B: amobarbital treatment partially restored the ECSH1 content compared with untreated hearts. Subunit 4 of cytochrome c oxidase (COX) was used as protein loading control. Background intensity was adjusted for the entire membrane in the membranes reprobed for subunit 4 of COX. Data are means ± SE; n = 3 in each group. *P < 0.05 vs. the time control or amobarbital-treated mitochondria; †P < 0.05 vs. the time control.